动物造模,鹌鹑痛风模型 z-bio 2021-11-11 322

　　Purpose: By simulating clinically high-protein and high-calcium diets to induce gout in quails, how to establish a preliminary study of quail gout models, and whether the research and prevention of the etiology of gout provide evidence for drug screening?

　　Method: Thirty-six 30-day-old Defark quail males were selected, and they were randomly divided into three groups according to their body weight and blood uric acid level: control group, type I group and type II group. The quail group was fed a basic diet, and the model group was fed with high protein and high protein. Calcium diet (the crude protein content in the diet is 29.5%, the calcium content is 7.8%), the control group? The model I group drinks free water, and the model II group restricts drinking water for 4 hours a day. Can the experiment last for 50 days? Simultaneously detect quail serum, urine and uric acid levels to detect kidney function and observe histopathological sections?

　　Result: High-protein and high-calcium diet can significantly increase the serum uric acid level of quail; significantly reduce fecal uric acid level; increase serum creatinine and urea nitrogen levels; cause severe uric acid deposition in quail kidneys? Will the combination of a high-protein and high-calcium diet and restricted drinking water aggravate the increase in blood uric acid levels? Will uric acid deposits in the kidney cause swelling, deformity and other symptoms of gout in certain quail joints? Is it more consistent with the clinical etiology, etiology and symptoms of gout, and provides reference materials for the basic knowledge of gout and drug development and research?