动物造模,支气管哮喘模型 z-bio 2021-10-27 515

　　Objective: To study the effects of different doses of ovalbumin (OVA) on serum IgE, IL-4, IFNγ levels, bronchoalveolar lavage fluid (BALF) and eosinophils (EOS) in a BALB/c mouse bronchial asthma model. A more suitable OVA model dose?

　　Method: The BALB/c mice were randomly divided into normal group, model 1 group (OVA20μg), model 2 group (OVA50μg), model 3 group (OVA100μg) and dexamethasone. Acetate (OVA 100μg)? Except for the normal group, are the mice in other groups sensitized by intraperitoneal injection of the corresponding dose of OVA and challenged with 1% OVA spray to establish a bronchial asthma model? ELISA to detect serum IgE? IL-4? IFN-γ content, Wright's staining can calculate the number of EOS in BALF, HE staining can observe the pathological changes of lung tissue?

　　Result: Compared with the normal group, the serum IgE of each group? IL-4 IgE? IL-4? IFN-γ significantly increased EOS in IFN-γ and BALF mice (P\u003c0.05). Inflammatory cells infiltrated lung tissue, bronchial epithelial cell degeneration, bronchial smooth muscle cell proliferation and other pathological phenomena have changed. Are the lung tissue content and pathological changes of the mice in the OVA 50μg group more obvious?

　　Conclusion: Three doses of OVA can successfully induce asthma in BALB/c mice. Generally, the asthma model induced by 50 μg OVA is more pronounced. Is BALB/c recommended as an appropriate model dose for a mouse asthma model?