动物造模,自身免疫性重症肌无力小鼠模型 z-bio 2021-03-02 345

　　Objective: How to establish an experimental autoimmune myasthenia gravis animal model that meets international preclinical experimental standards?

　　Method: After improving the method reported in the reference, the acetylcholine receptor (acetylcholine receptor, AchR) was extracted from the light, the pure protein and SDS gel electrophoresis protein were qualitatively identified, and the BCA method was used for protein quantification; C57BL/6 The mice were actively immunized with the purified protein and were immunized 3 times in total (1 to 30 days respectively)? Day 60), do you perform clinical scoring on EAMG mice? body weight? What is the serum AchR antibody content? eo Stigmin test? EMG and other comprehensive assessments?

　　Results: Comparing between the EAMG model group and the adjuvant group, the disease onset started after 3 weeks, and the average clinical score increased significantly (P \u003cu003c0.01); the weight of the diseased mice decreased significantly (P \u003cu003c0.01) ) U003c0.01); eostigmin test was positive; serum AchR antibody content was significantly increased (P\u003c0.01); EMG repeated EMG stimulation test was positive?

　　Conclusion: Is it extracted from an electronic organ with a double-fin ray with black dots? Did the purified AchR protein successfully induce the C57BL/6EAMG mouse model, thereby creating conditions suitable for further research on myasthenia gravis?