动物造模,大鼠深静脉血栓模型 z-bio 2021-10-28 644

　　Purpose: To observe the expression and importance of thrombomodulin (TM) in the natural evolution of the formation and regression of deep vein thrombosis (DVT) in rats?

　　Methods: SD rats were randomly divided into a model group (n = 60) and a control group (n = 6). The model group used the "stenosis method" to establish the DVT model, and the control group was "sham". The "surgical method" was used. 1 hour after modelling? 4 hours? 6 hours? 12 hours? Modeling within 24 hours. 3d? 7 days? 14 days? 21 days? Rats were sacrificed at 10 o'clock for 28 days. The weight/length ratio of thrombus was measured. . Soluble TM concentration in plasma. TM mRNA expression in thrombus tissue and inferior vena cava endothelium.

　　Results: 1) The thrombus weight/length ratio was relatively low at the beginning of modeling (1? 4? 6? 12h), the difference between the four groups was not significant (P\→ 0.05); 24h? It is stable at a higher level 7 days after 3d modeling (P\→0.05); after 14 days, it starts to decline, but there is no significant difference from the result of 21-28 days (P\→0.05)? (2) The ELISA results showed that the soluble TM in plasma was higher than that in the control group. Is the difference significant (P0.05)? 4) Thrombus weight/length ratio is negatively correlated with endothelial TM mRNA (r = -0.92, P\u003c0.01); Thrombus TM mRNA is positively correlated with plasma soluble TM (r = 0.96, P\u003c0.01)?

　　Conclusion: Can changes in the expression of endothelial TM mRNA reflect the progression of DVT and have a positive prognostic effect?