动物造模,结肠癌肝转移裸鼠模型 z-bio 2021-11-11 569

　　Objective: How to establish a colon cancer liver metastasis model with high metastasis rate? Is the operation simple? Reliable results of experimental studies on the prevention and treatment of colon cancer metastasis?

　　Methods: 15 Balb/c nude mice were divided into three groups (group A (group B, group C), and 5 Balb/c mice were divided into group D. The colon was constructed by spleen transplantation and preservation of the spleen. It is 2.5×107/mL 0.2 mL HCT116CT26 cell suspension model cancer liver metastasis model, success rate and liver metastasis size. Do you want to compare the four groups of animal models? Number and intraperitoneal metastasis?

　　Results: The success rate of the nude mouse model in group A was 100% (5/5). All liver and spleen formed tumors, liver metastases, few, scattered, and mostly distributed in the right lobe of the spleen. The average survival time was (26.6±). 3.4) Day, the success rate of nude mice in group B was 40% (2). 5) The metastatic tumors were scattered on the surface of the liver, the volume was larger than that of group A, and the average survival time was (36.8±4.2) days; the success rate of nude mice in group C was 100% (5/5). The average survival time of the entire right lobe of the liver was (20.2±2.6) days; no metastasis was found in the spleen of group D; 3 groups of nude mice (group A2, group C3) had abdominal metastasis, no colon, and 3 groups of lung nude mice had liver metastases Does the histological morphology match the characteristics of adenocarcinoma?

　　Conclusion: The preservation of the spleen can achieve a higher model success rate, and effectively reach the method and process of the human colon.