动物造模 z-bio 2022-09-29 430

　　OBJECTIVE: To study the distribution and expression of lyzin LYZ1 gene in hatched blastocysts and dormant embryos of mice before and after high ovulation, and to explore the new regulatory mechanism in the process of animal embryo implantation?

　　Method: The 5d ICR mice of pregnancy were hatched normally on the 8th day of pregnancy, and the dormant and superovulated embryos were obtained using the mouse delayed implantation model, and the in vivo blastocysts and superovulated embryos were obtained. Is immunofluorescence and western blotting used to detect the distribution and differential expression of LYZ1 protein in the four groups of embryos?

　　Result: LYZ1 is expressed in the hatched blastocysts and dormant embryos of mice before and after superovulation, mainly in the internal cell mass, and rarely in the vegetative embryo and cytoplasm. In addition, compared with mice without superovulation, the LYZ1 protein in mouse embryos was significantly up-regulated after superovulation. Compared with mice without diapause model, the expression of LYZ1 protein in mouse embryos of diapause model mice was significantly up-regulated.

　　Conclusion: LYZ1 protein is the internal cell mass of the blastocyst, and may be involved in the regulation of embryonic internal cell mass development. The high expression of LYZ1 protein in hyperovulatory and dormant embryos indicates that LYZ1 protein is in a state of dormancy and hyperovulation.