动物造模 z-bio 2021-12-27 361

　　Objective: How to find specific indicators of spleen, kidney, and renal failure?

　　Method: 96 Wistar rats were randomly divided into model group, high-dose group, middle-dose group, low-dose group and SASP group. The corresponding drugs were given to the treatment group. Mandatory oral? Have blank and model colon tissues been selected for high-throughput sequencing? T? Do you use qPCR methods to detect and screen the expression of chemokine genes?

　　Result: On the contrary, whether to screen the differentially expressed genes in the blank group according to the q value ≤ 0.0 fold change ≥ 1.5. The analysis of GO function classification shows that the differential gene function is mainly enhanced by biological process (BP). Cellular components (cell component (CC) → molecular function (MF) 3 levels). In the enrichment analysis of differential genes KEGG, the expression of CXCL1, CXCL2, CXCR2, CXCL6, CCL7 and CCL12 genes in the chemokine signaling pathway is very important. The gene expression changes of the above factors are consistent with the sequencing results, confirming that the expression of the above factors is significant after treatment with the spleen Benshen Recipe.

　　Conclusion: CXCL1? The expression of CXCL2, CXCR2, CXCL6, CCL7, and CCL12 genes in the chemokine signaling pathway of ulcerative colitis with weak spleen and kidney were significantly up-regulated. UC can be used as an objective indicator of mucosal inflammation, and the chemically bound particles of Rizontan and Sishenwan have therapeutic effects. Can strengthening the spleen and kidney effectively down-regulate the expression of the above factors, delay the inflammatory response, and promote the repair of the damaged colonic mucosa?