动物造模,缺氧缺血性脑病 z-bio 2021-12-27 642

　　Objective: To investigate the effect of edaravone on cerebral edema and CD163/HO-1 signaling pathway in neonatal rats with hypoxic-ischemic encephalopathy (HIE)?

　　Methods: 120 7-day-old SD rats, 90 of them were double ligated, and hypoxic-ischemic brain injury (HIBD) models were established with common carotid artery and hypoxia. They were randomly divided into model group and edaraben group. The remaining 30 were only threaded threads, not ligated, and hypoxia was used as a sham operation group. After the model was established, edaravone 2 mg/kg was injected intraperitoneally into the edaravone group immediately for 5 days, and the same dose of normal saline was injected intraperitoneally into the model group and the sham operation group at the same time every day. Pay attention to the biological behavior of SD newborn rats in each group. TTC staining can observe the brain tissue morphology of each group of SD neonatal rats 24 hours after modeling. After modeling, each group was 6 hours, 12 hours, 24 hours, 2 days, 3d and 5d. Changes of brain water content in SD neonatal rats; CD163? Fluorescence quantitative PCR (qRT-PCR) technology for detecting the expression level of HO-1 mRNA; CD163? Does Westernblot technology detect HO expression level 1 protein?

　　Result: 90 SD neonatal rats showed symptoms of lethargy, lethargy and convulsions after the operation. After edaravone treatment, the symptoms were significantly reduced compared with the model group and improved within 3 days. The left hemisphere of the newborn rats in the TTC stained model group was found to have edema and light color 24 hours after modeling. Its volume is slightly larger than the right hemisphere, and the degree of brain tissue damage is significantly reduced after edaravone treatment. In the model group, the infarct area of the left hemisphere of neonatal rats in the edaravone group was significantly larger than that in the sham operation group (P\u003c0.05). The area of cerebral infarction after Ray-Ban was significantly smaller than the model group (P\u003c0.05). The results of brain tissue water content test showed that the brain tissue water content of newborn rats in the model group and edaravone group was significantly higher than that in the sham operation group. 6 hours after modeling. In this group (P\u003c0.05), is the brain water content the highest in 2 days? After edaravone treatment, the brain tissue water content of newborn rats was significantly lower than that of the model group (P\u003c0.05). The results of qRT-PCR and Westernblot showed that they were compared with the sham group. In contrast, the expression levels of CD163 HO-1 mRNA and protein in the brain tissue of neonatal rats in the model group edaravone group were significantly increased (P\u003c0.05). ), CD163 HO-1 mRNA and protein expression in neonatal rats increased significantly after edaravone treatment. Is the protein expression level significantly higher than the model group (P\u003c0.05)?

　　Conclusion: The mechanism by which edaravone can significantly reduce brain edema and cerebral infarction in neonatal rats with HIBD may be related to the activation of the CD163/HO-1 signaling pathway?