动物造模,大鼠外周痛感觉调控机制 z-bio 2021-11-23 395

　　Objective: To observe the relationship between TRPV1 and P2X3 in peripheral rats, and to clarify part of the sensory regulation mechanism of peripheral pain?

　　Method: A blank control group of male SD rats randomly? TRPV1 agonist group? Divided into P2X3, agonist group? TRPV1 agonist + P2X3 agonist group? TPRV1 agonist + P2X3 inhibitor group? P2X3 agonist + TRPV1 inhibitor group? TRPV1 or P2X3 agonists and/or inhibitors were injected subcutaneously into the soles of the feet, and the rats in each group were observed. It shrinks in 20 minutes. Number of legs? Time to lift/lick the leg; use immunofluorescence to observe the expression and co-expression of TRPV1 and P2X3 positive regions at the level of L4DRG; observe the co-expression of TRPV1 and P2X3 at the level of L4DRG by immunoprecipitation. Do you do this?

　　Results: P2X3 excitatory P2X3 inhibitors cannot improve the pain behavior caused by TRPV1 agonists; P2X3 inhibitors can reduce the pain behavior caused by TRPV1 agonists; TRPV1 agonists can increase the pain behavior caused by P2X3 agonists, but TRPV1 inhibitors cannot Relieve painful behaviors. Caused by P2X3 agonists. P2X3 agonists can increase the expression of TRPV1 positive regions at L4DRG levels, and TRPV1 agonists can increase the expression of P2X3 positive regions at L4DRG levels. TRPV1 and P2X3 are combined. -Is it expressed at the DRG level and has co-precipitation?

　　Conclusion: At the level of peripheral neurons, is there a specific interaction between TRPV1 and P2X3? Can the two promote each other's expression? If one of them is blocked, will the function of the other be reduced accordingly?