动物造模,醒脑静注射液 z-bio 2021-10-28 431

　　Objective: To observe the dose-effect relationship of Xingnaojing injection in Beagle dogs coma.

　　Methods: 42 male beagle dogs weighing 8-10kg were randomly divided into normal control group, model control group, Xingnaojing injection group 1 (0.43mL/kg), dose group 2 (0.86mL/kg), dose . Enter 3. Double-dose group ((0.43 + 0.43) mL/kg), dose 4 group (1.29 mL/kg), dose 5 double-dose group ((0.65 + 0.65) mL/kg), Goulumianning (0.02 mL) /kg) kg) is used for anesthesia and fixed with a homemade percussion instrument. The percussion device was placed at 150 cm, perpendicular to the back of the dog’s head, and struck the dog four times in succession to establish a traumatic brain coma model. Immediately after modeling, Xingnaojing injection was given in different doses, the dose was 10.0 mL/kg, the recovery time was observed, and then brain MRI was performed. MRI examination was performed 14 days after administration. , And take blood to check blood routine, liver and kidney function, serum S-100 calcium binding protein (S-100B) and neuron-specific enolase (NSE) content. For histopathological examination, the heart, liver, spleen, lung, kidney, adrenal gland and brain of each group of animals were taken.

　　Result: The dose 1, dose 2 (single dose), and dose 3 (double dose) of Xingnaojing injection can significantly shorten the recovery time of dogs. 1 dose of Xingnaojing, 2 doses of Xingnaojing and 4 doses of Xingnaojing can significantly reduce serum S100B and NSE levels. Xingnaojing dose 1, dose 2, dose 3 (2 doses), dose 4, dose 5 (2 doses) can significantly increase the damage recovery area detected by canine brain MRI (P\u003c0).05). Xingnaojing injection group 1 and 2 can significantly reduce the number of conventional blood lymphocytes and eosinophils; Xingnaojing injection group 1 and 2 can significantly reduce the number of canine monocytes; Xingnaojing injection The 4th and 5th pairs of doses have no obvious effect on the blood routine of dogs. There was no significant difference in liver and kidney function indexes (ALT, AST, TBil, BUN, CRE) between the Xingnaojing injection groups. The histopathology of the heart, liver, spleen, lung, kidney and brain organs of each group of animals was not significantly different.

　　Conclusion: Xingnaojing injection can significantly improve the degree of coma and brain damage caused by brain trauma in Beagle dogs, and there are no obvious side effects when used in large doses.