动物造模,大鼠心肌缺血再灌注损伤 z-bio 2021-12-28 348

　　Objective: To study the protective effect and mechanism of activated protein C (APC) on myocardial ischemia-reperfusion injury in rats.

　　Method: 30 male SD rats were randomly divided into sham operation group (10), I/R group (10) and APC treatment group (10). The left anterior descending artery was ligated for 60 minutes and reperfused for 6 hours. The myocardial ischemia-reperfusion model was established, and the expression levels of TNF-α, myocardial tissue MPO and ICM-1 were detected by ELISA and immunohistochemical methods, and the pathological changes of myocardium in each group were detected by HE staining.

　　Result: Compared with the sham operation group, the expression levels of TNF-α, MPO and ICM-1 in the sham operation group were significantly increased (P\u003c0.01). Compared with the I/R group, the expression levels of α, MPO and ICM-1 in the TNF-αAPC group were significantly reduced (P\u003c0.01), and inflammatory cell infiltration was observed under an optical microscope. The I/R group was the most prominent. Compared with the IR group, the APC group was significantly lower.

　　Conclusion: APC has a protective effect on myocardial ischemia-reperfusion injury, and its mechanism may be related to inhibiting the expression of inflammatory factors and reducing tissue inflammatory cell infiltration.