动物造模,肥胖小鼠 z-bio 2021-10-29 270

　　Objective: To perform hypoxic training on obese mice to observe the changes in gastric ghrelin and hypothalamic GHSR levels to determine whether hypoxic training affects the GHSR pathway to improve obesity.

　　Method: Male C57BL/6J mice were randomly divided into a normal control group (C, n = 8) and a high-fat diet group (H, n = 52). After the obesity model was established in group H, they were randomly divided into obesity control group (HC), obesity normoxic exercise group (HE), obesity hypoxic exposure group (HH) and obesity hypoxic exercise group (HHE). ). Each exercise group received moderate-intensity treadmill training, each hypoxic group had intermittent hypoxia (oxygen content of 11-2%), weekly weight and 4 weeks of food intake records, serum, TC, TG, GLU and total cholesterol were tested for grelin levels, RT-PCR was used to detect hypoxic GHSR and gastric GhrelinmNA expression levels, WB was used to detect the levels of GHSR and NPY protein in the thoracic cavity and the levels of grelin, goat and HIF_2α proteins in gastric tissue.

　　Results: (1) After 4 weeks of intervention, the weight of HE, HH and HHE groups was significantly lower than that of HC group. In the early stages of hypoxia intervention, the HH and HHE groups had higher food intake. Despite the decrease, with the extension of the intervention time, the food intake of each obesity group gradually approached. (2) The blood index of HC group was significantly higher than that of C group. The TC level in the HH group was significantly higher. The GLU level of HE, HH and HHE group was significantly lower than that of HC group. The total serum ghrelin level of the HC group was significantly lower than that of the HC group. 3) The levels of hypothalamic GHSRMRNA and gastric ghrelin mRNA in HC group were significantly lower than those in C group. 4) Hypoxic GHSR protein levels in HE, HH and HHE groups were significantly higher than those in HC and NPY groups. The protein level of HE and HHE group was significantly higher than that of HC group. (5) The protein level of ghrelin in the HE, HH and HHE groups was significantly higher than that of HC. HE and HHE goat protein levels were significantly higher than HC group, HH2α protein levels in HH and HHE groups were significantly higher than HC group.

　　Conclusion: Hypoxic training can affect the metabolism of glucose and lipids by regulating the GHSR signaling pathway, thereby reducing the weight of obese mice.