动物造模,脐带间充质干细胞 z-bio 2021-10-22 305

　　OBJECTIVE: To investigate the effects of umbilical cord mesenchymal stem cells on the expression of sex hormones, CYR61 and CTGF in immature rabbits with ovarian failure, and to clarify their therapeutic effects and potential mechanisms.

　　Method: 10 rabbits were randomly divided into model group, 5 rabbits were divided into treatment group, all rabbits were injected intraperitoneally with CTX (50mg/(kg.d) for 2 days) to establish a POF model. I injected it into the treatment group. UCMSC was injected into the ear vein (5 x 106/mL, 2 mL each time, for 3 consecutive days), and the same amount of sterile water was injected into the model group and venous blood after 0, 7, 14 and 28 days. The treatment of UCMSCs and changes in serum hormone levels were analyzed. 28 days after treatment, samples of ovarian tissue were taken to detect the expression of CYR61 and CTGF protein and mRNA in ovarian tissue.

　　Results: 1. The serum E2 and FSH levels between the model group and the treatment group before treatment were not different and comparable (P \u003c0.05). After treatment, the rabbit serum E2 level increased, and the FSH level increased. Although the rabbits in the model group have higher E2 content, their FSH levels are higher. Although they are lower as in the model group, the difference is statistically significant (P\u003c0.05), and it changes periodically over time, and there is no periodic fluctuation . Fluctuating over time. 2. Compared with the model group, the expression of CYR61 and CTGF protein in the treatment group was significantly increased, and the difference was statistically significant (tCYR61 = 8.68, P\u003c0.05, tCTGF = 10.142, P\u003c0.05). 3. The expression levels of CYR61 and CTGF mRNA in the treatment group were significantly higher than those in the model group, and the difference was statistically significant (tCYR61 = 38.156, P\u003c0.05, tCTGF = 14.646, P\u003c0.05). 4. CYR61 and CTGF are mainly expressed in granulosa cells and follicular cell membranes. Compared with the model group, the expression of the treatment group increased significantly, and the difference was statistically significant (tCYR61 = 3.981, P\u003c0.05, tCTGF = 4.111, P\u003c0.05).

　　Conclusion: Umbilical cord mesenchymal stem cell therapy can improve sex hormone levels in POF rabbits, up-regulate the expression of CYR61 and CTGF, thereby activating the formation of dormant follicles, restoring ovarian function, and bringing therapeutic effects to POF.