动物造模,慢性肝损伤小鼠 z-bio 2021-10-22 406

　　Objective: To study the antioxidant protection of aquatic turtle peptide on CC14-induced chronic liver injury in mice.

　　Method: The mice were randomly divided into normal group, model group, tortoise peptide group (50, 100, 200 mg/kg) and positive drug group. The mice in the normal group were given normal saline, while the mice in the tortoise peptide group were given different saline. Mice in the tortoise ground peptide and positive drug group were given silymarin (100 mg/kg) daily for 6 weeks. At the same time, mice in the normal group were injected intraperitoneally with peanut oil solution twice a week 24 hours after the last dose, and the other groups were injected intraperitoneally with 10 mL/kg of 0.1% CC14 peanut oil solution. The mice were weighed for 6 weeks, and liver tissue was collected at the expense of expense. The histological changes of the liver are as follows: Observe by HE staining, and detect liver function enzymes (AST, ALT) and antioxidant enzymes (SOD, SOD, SOD, etc.) by spectrophotometry. CAT, GSH-Px, peroxide (MDA), ELISA gene expression was measured by quantitative fluorescent PCR method to determine the protein levels of inflammatory factors, apoptosis factors, fibrosis factors and inflammatory factors.

　　Result: Through microscope observation, no abnormality was found in the liver cells of the normal group. The liver cells in the model group showed congestion, necrosis, fibrosis and loss of liver lobules. The mouse peptide group was destroyed. The increase in the degree of liver cell damage was significantly reduced. The liver and spleen index of mice was significantly lower than that of the model group. Compared with the model group, serum AST and ALT activities were higher. The proportion of mice in the tortoise peptide group was significantly reduced, liver MDA content was significantly reduced, anti-apoptotic enzyme (SOD), CAT, GSH-Px) activity, inflammatory factors (IL-6, TNF-α, iNOS) The protein increased significantly and the gene expression level decreased significantly, and the gene expression of apoptosis promoting factor (Bax, caspase-3) and fibrosis factor (α-SMA, TGF-β) also decreased significantly.

　　Conclusion: The earth effect peptide can effectively reduce the chronic liver injury in mice caused by CC14, and its protective effect may be closely related to the antioxidant effect of the earth effect peptide.