动物造模,脑缺血再灌注损伤 z-bio 2021-03-04 269

　　Objective: To investigate the effects of zedoary ketone on cognitive function, neurological function and cerebral ischemia-reperfusion injury in mice.

　　Methods: Using the suture method, establish a mouse cerebral ischemia-reperfusion injury model, low molecular weight heparin sodium group, low-dose and high-dose turmeric dione group, and 1mg/(kg.d), respectively after the model is established Take 20 mg. Observation/(kg.d), 60 mg/(kg.d), Morris water maze test, behavior score, cerebral infarction rate, brain tissue water content of each group of mice, serum 6-keto-PGF1α and 6-keto -determine. PGF1α/TXB2 ratio, the expression level of cAMP and p-CREB in brain tissue homogenate. Compared with the sham operation group, the escape latency, platform crossing times (P\u003c0.01), behavior score, cerebral infarction rate, brain water content and serum TXB2 content of the model group were significantly prolonged. decrease very much. Serum 6-keto-PGF1α, 6-keto-PGF1α/TXB2 ratio, brain homogenate cAMP and p-CREB increased significantly (P\u003c0.01), but significantly decreased (P\u003c0.01). Compared with the model group, the escape latency of the turmeric 2 ketone group was significantly shortened (P\u003c0.05), the number of platform crossings (P\u003c0.05) was significantly increased, and the behavior score was significantly increased. Water content was significantly reduced (P\u003c0.05), serum TXB2 content was significantly reduced (P\u003c0.01), serum 6-keto-PGF1α, 6-keto-PGF1α/TXB2 ratio, brain homogenate cAMP and p- CREB was significantly increased (P\u003c0.05), and it was dose-dependent.

　　Conclusion: Gajutsuketone has an excellent protective effect on the cognitive and neurological function of mice with cerebral ischemia-reperfusion injury. Its mechanism is to improve the microcirculation disorder and cAMP/CREBβ/BDNF signal pathway, which may be related to activation.