动物造模,狼疮小鼠脾淋巴细胞 z-bio 2021-03-04 389

　　Objective: To observe the effect of hydroxychloroquine on the distribution of lymphocytes in the peripheral immune system (spleen) of mice with systemic lupus erythematosus (TC mice).

　　Method: 30-week-old female TC mice were randomly assigned. The experimental group was given hydroxychloroquine through forced feeding [10 mg/(kg·D)], and the control group was given normal saline through forced feeding [10 mg/(kg)]. ? d)], a total of 5 weeks of treatment. Enzyme-linked immunosorbent assay (ELISA) was used to detect mouse peripheral blood anti-dsDNA antibody titers, hematoxylin-eosin (HE) staining was used to detect the pathological changes of kidney tissue, and flow cytometry was used to detect the proportion of mouse spleen.

　　Results: Compared with the control group, the renal histopathological score of the experimental group was significantly lower than that of the control group (P0.05).

　　Conclusion: Hydroxychloroquine has a regulatory effect on the immune response of lymphocytes in the peripheral immune system of lupus mice, and can reduce the pathological damage of TC mice to the kidney tissue.