动物造模,抗肿瘤作用 z-bio 2021-03-04 532

　　Objective: To study the anti-tumor effect of Xiuzhen mushroom and its mechanism.

　　Method: Choose 60 ICR male mice and 10 as the blank group, and subcutaneously inoculate the remaining 50 mice with H22 mouse ascites to establish a tumor-bearing mouse model. After modeling, they were randomly divided into model group and positive. There were 10 animals in the drug (CTX) group, the low, medium and high dose groups of Xiuzhen mushroom. The mice in the model group were given normal saline, and the mice in the positive group were injected intraperitoneally with 20 mg/kg body weight of cyclophosphamide (CTX) normal saline every other day. The mice in the dose group and high-dose group were 750 and 1500, respectively. Xiuzhen mushroom physiological saline suspension 3000 mg/kg body weight. After 10 days of administration, the average tumor weight and tumor suppression rate of each group of mice were measured, immune organ index, serum immunoglobulin and cytokine content, spleen and kidney antioxidant index, and tumor and HE staining to measure the pathology of spleen tissue . A slice is observed.

　　Results: (1) The average tumor weight of mice in the CTX and Xiuzhen mushroom low, medium, and high dose groups was significantly lower than that of the model group (P\u003c0.01), and the tumor inhibition rate for mice was higher. 4 groups are 55.18. % And 29.06%, 47.47% and 48.80%.

　　(2) Compared with the blank group, the spleen index, serum IgA and TNF-α content and liver MDA content of the model group were significantly increased (P0.05), serum IL-2 content and liver. GSH-Px activity and renal SOD, GSH-Px and CAT activities were significantly reduced (P\u003c0.05, P\u003c0.01). At the same time, the thymus index of the CTX group was significantly lower than that of the blank group (P\u003c0.05, P\u003c0.01). P\u003c0.05).

　　(3) Xiuzhen mushroom has a reversal effect on the abnormal changes in immune and antioxidant function of tumor-bearing mice: the serum IgA and TNF-α levels of mice in each dose group of Xiuzhen mushroom are compared with those of the model group and the plasma of mice in the high-dose group. The level of IL-2 was significantly increased (P\u003c0.05, P\u003c0.01), and the serum IL-2 level of mice in the low-dose group was significantly increased (P\u003c0.01). Xiuzhen mushrooms. The treatment also significantly improved the liver CAT activity of tumor-bearing mice in each dose group, including renal SOD and CAT activity (P\u003c0.05, P\u003c0.01), and liver and kidney GSH in the middle and high-dose groups. GSH-Px activity-Px activity in the low-medium-dose group (P\u003c0.05, P\u003c0.01), the liver MDA content of the middle-dose group was significantly reduced (P\u003c0.05, P\u003c0.01) u003c0 .05).

　　(4) Compared with the CTX group, the spleen index of the mice in the Xiuzhen mushroom high-dose group was significantly increased (P\u003c0.05), and the serum IL-6 level was significantly reduced (P\u003c0).05).

　　(5) The histopathological section of the tumor showed that the area of tumor necrosis was significantly increased in the CTX group and the Xiuzhen mushroom each dose group.