动物造模,黑色素瘤肺 z-bio 2021-11-04 657

　　Objective: To investigate the effect of atrial natriuretic peptide on pulmonary metastasis of melanoma.

　　Method: The melanoma B16F10 cell line cultured in vitro was injected into atrial natriuretic peptide knockout mice (AP-/- mice) and C57BL/6J mice through the tail vein to establish a melanoma lung metastasis model. Establish a model, count the number of nodules transferred to the surface of the lung, implant and fix the lung tissue, slice and stain with HE to identify and count the lung metastasis nodules.

　　Results: The number of metastatic melanoma nodules on the lung surface of AP knockout mice was significantly lower than that of C57BL/6J mice, the difference was significant; the number of lung micrometastasis nodules in AP knockout mice was also significant . The number of C57BL/6J rats is significantly reduced, and the difference is large.

　　Conclusion: AP gene knockout significantly inhibited the lung metastasis of melanoma.