动物造模,小鼠肾脏 z-bio 2021-03-05 458

　　Objective: To investigate the regulation effect of Huangqi injection on immune cells and inflammatory factors in db/db mice and its possible mechanism.

　　Method: C57BL/6 mice were used as a control group, and dbdb mice were randomly divided into a diabetes group and a diabetic xanthosis injection treatment group. The mice in the control group and the diabetes group were injected intraperitoneally with 100 μL of PBS per day, and the mice in the diabetes ranger injection treatment group were injected with 100 μL of ranger every day for 12 weeks. After 12 weeks, the number and classification of peripheral blood white blood cells were measured. ELISA method was used to detect the expression of interleukin 10 (IL-10) and tumor necrosis factor α (TNF-α) in serum. The expression of interleukin 1β (IL in mouse kidney) was detected by real-time PCR. -1β), TNF-α and human monocyte chemoattractant protein 1 (MCP-1) mRNA expression; flow cytometry was used to detect the proportion of regulatory T cells (Treg) in the spleen.

　　Result: The peripheral blood white blood cell count, neutrophil ratio and monocyte ratio of the diabetes group were significantly higher than those of the control group, and the ranger injection treatment group was significantly lower than the diabetes group. group. The serum II-10 of the diabetes group was significantly lower than that of the control group, and TNF-α was significantly higher than that of the control group. The level of IL-10 in the astragalus injection treatment group was significantly lower than that of the diabetes group, and the level of TNF-α was significantly lower than that of the diabetes group. The expressions of IL-1β, MCP-1 and TNF-α in the diabetic group were significantly higher than those in the control group, and the expressions of IL-1β, MCP-1 and TNF-α in the astragalus injection treatment group were significantly lower than those in the diabetic group. The diabetes group was higher than the control group, but the ranger injection treatment group was significantly higher than the control group.

　　Conclusion: Astragalus injection can reduce the number of inflammatory cells in db/db mice, increase the number of Tegs cells, and reduce the expression of pro-inflammatory factors IL-1β, MCP-1 and TNF-α.