动物造模,大鼠褪黑素 z-bio 2023-01-13 807

　　Objective: To observe the effect of ozone autoblood on melatonin and oxidative stress indicators in rats with insufficient sleep.

　　Method: We selected 40 adult male Wistar rats and used the "modified multi-platform sleep deprivation method" to create a REM sleep (REM) sleep deprivation model. According to the principle of the random number table, the rats were divided into a control group and an observation group, with 20 rats in each group. The rats in the observation group received ozone autotransfusion, and the rats in the control group were controlled by air. Serum melatonin levels were detected by collecting tail vein blood at 0, 24, 48, and 72 hours. Superoxide dismutase (SOD) and glutathione were detected at 0 and 72 hours, which are indicators of venous blood oxidative stress. Peroxidase (GSH-px) and malondialdehyde (MDA) content.

　　Result: For the 0 hour intervention, the difference between the two groups of awake rats (wake W), slow wave sleep (slow wave sleep, SWS) and fast wave sleep (fast wave sleep, FWS) is as follows: The difference was not statistically significant (P\→0.05); 72 hours after the intervention, W in the observation group was lower than the control group, while SWS and FWS were higher than the control group (P\u003c0.05). After 48 hours and 72 hours of intervention, the serum melatonin level of the observation group was higher than that of the control group (P\u003c0.05). After 72 hours of intervention, the SOD and MDA levels of the observation group were lower than those of the control group, and the GSH-px level was higher than that of the control group (P\u003c0.05).

　　Conclusion: The ozone autoblood method can promote the release of melatonin in sleep-deficient rats, reduce the serum SOD and MDA content in rats, and increase the serum GSH-px content in rats.