动物造模,导型关节炎大鼠 z-bio 2021-11-01 554

　　Objective: To investigate the effect of Linagliptin on extracellular regulatory protein kinase (ERK) pathway in collagen-induced arthritis rats.

　　Method: 30 SPF male SD rats were randomly divided into control group, model group and rigstradin group, each with 10 rats. The collagen-induced arthritis model was administered to rats in the model group and the ligstradin group. After the model was successfully established, rigstradin (0.1 g/kg) was administered to the rigstradin group daily for 28 days, and then to the model group and the normal group.. each Daily use strastradine (0.1 g/kg) equal amount of normal saline. The volume changes of the hind legs of the three groups of rats were measured every week, the infiltration of inflammatory cells in each group was observed by HE staining, and the tumor necrosis factor α in the synovial tissue and serum of the rats was detected in real time. Time fluorescence. Quantitative PCR and ELISA (tumor necrosis factor α, TNF-α), angiopoietin 1 (angiogenin 1, Ang-1), interleukin 1β (interleukin 1β, IL-1β) and vascular endothelial growth factor ( Blood vessel) endometrial growth factor, VEGF) the level and expression level of ERK pathway related proteins in mouse synovial tissue.

　　Result: Starting from 2 weeks after modeling, the hind paw volume of the model group was significantly larger than that of the control group (P\u003c0.05), and the hind paw volume of the rigstragin group was larger than that of the model group. Also greatly reduced. At the same time point (P\u003c0.05); at the same time, the expression levels of inflammatory factors TNF-α, Ang-1, IL-1β and their mRNA in the serum of the model and synovial tissue and the control group (P \u003c0.05) The level of VEGF in synovial tissue in the ligstradin group was lower than that in the model group (P-0.05), and the phosphorylated ERK1/2 (p-ERK1/2) protein expression model. The expression level of p-ERK1/2 protein in the regattazine group was significantly lower than that in the model group (P\u003c0.05), although both this group and the ligstradin group increased (P\u003c0.05).

　　Conclusion: Repagliptin can effectively block the ERK pathway, regulate the levels of TNF-α and IL-1β in the serum and synovial tissue of collagen-induced arthritis rats, and reduce the symptoms of joint swelling.