疾病动物模型,大鼠脑瘫 z-bio 2021-11-06 229

　　1. Experimental cerebral palsy animal model after asphyxia in newborn rats:

　　Newborn rats underwent ligation of the right common carotid artery under awake local anesthesia, and then placed the animal in a constant temperature airtight glass container with a mixture of 8% oxygen + 92% nitrogen, and took it out 2 hours later. Observe the young rat Performance, and then grouped and decapitated.

　　2. Rat central spastic paralysis model:

　　Randomly divide the above-mentioned upper limb spastic cerebral palsy rats into 2 groups according to the operation sequence, with 8 rats in each group. Experimental group: C6 nerve roots were cut off.

　　3. Improvement of rat spastic cerebral palsy model:

　　Wister rat, male or female, 200-250g, pentobarbital 30mg/kg intraperitoneal anesthesia. A longitudinal incision was made at the sagittal suture at the top of the skull to expose the bregma. The modified de groot positioning system was used to select 6.6 mm behind the bregma and 1 mm to the left of the sagittal suture. Use a small electric drill to drill a hole on the skull plate with a diameter of approximately 2mm, the anode needle electrode, about 0.5mm in diameter, is inserted vertically in 9.7mm, the cathode is connected to the tail of the mouse, and direct current stimulation is applied to destroy the vertebral body bundle. After stimulation, the electrode is pulled out, the incision is closed, the rat is immediately killed, and the specimen is taken.