疾病动物模型,家兔心肌病模型 z-bio 2021-03-09 252

　　[Modeling mechanism] Injecting large amounts of norepinephrine into rabbits will increase myocardial load, myocardial degeneration and necrosis, and may cause DCM and congestive heart failure.

　　[Modeling method] Japanese big-eared white rabbits are about 6 months old, and the weight of hermaphrodite is 2.1-2.5 kg. The animals were fixed in a rabbit retention cage without anesthesia, 1 mg/kg of norepinephrine was intravenously infused into the model group, norepinephrine was added to 40 ml of saline, and 90 minutes from the ear vein Complete the infusion within. The control group only received intravenous injection of saline, and the infusion rate and body fluid volume were the same as the model group. Collect experimental data and samples after 15 consecutive doses. After the model is made, open your chest to get the material.

　　[Model Features] The calcium ion content of peripheral blood erythrocytes of this model animal is significantly higher than that of the control group, while the magnesium ion content is significantly lower than that of the control group; the calcium ion content of myocardial tissue is higher than that of the control group. Significantly lower than the control group, significantly higher than the control group, and the Mg2 + content was significantly lower than the control group. In some animals, the heart of the model animal may become larger and softer, the color of the myocardium may darken, and pericardial effusion may appear. Under an optical microscope, the myocardial tissue is flaky or punctate necrosis, myocardial fibers are swollen and destroyed, some necrosis and fusion, capillary exudation, microthrombosis, some myocardial cell vacuole degeneration, nuclear enlargement and deep staining. Under the electron microscope, the mitochondria were obviously swollen, the cr membrane was blurred, myofibrils were misaligned, contraction, destruction and vacuoles were serious. Glycogen particles are significantly reduced and fat droplets are reduced. Most myoplasmic reticulum tissues have increased and extensive changes, chromatin nuclei are concentrated, secondary lysosomes are increased, myocardial capillary edema, and a large number of hydrolyzed vesicles in the cytoplasm. In addition, model animals are also associated with significant hemodynamic changes.

　　[Model Evaluation and Application] The norepinephrine-induced rabbit DCM model has the advantages of easy modeling, short time and clear pathological changes. It is especially suitable for dilated cardiomyopathy and congestive heart failure caused by catecholamine metabolism disorder.