疾病动物模型,心绞痛动物模型 z-bio 2021-03-09 329

　　[Modeling mechanism] Pituitary prostaglandin will severely contract the coronary arteries, cause acute dysfunction of the blood supply of myocardial tissue, increase peripheral resistance, increase the burden on the heart, myocardial ischemia, hypoxia and even necrosis, which may cause.

　　[Modeling method]

　　Rabbit: Place the animal in the supine position of the intervention console and connect it to the ECG device (excluding animals with abnormal ECG). Pituitrin was injected into the ear vein at a dose of 1.5-2.5 U/kg. The injection was completed within 30 seconds after 0 seconds, 5 seconds, 10 seconds, 15 seconds, 30 seconds, 1 minute and 2 seconds. The injection is over. Record the ECG in minutes, 5 minutes, 15 minutes and 20 minutes. After successful modeling, the rabbit ECG is divided into two stages. The first stage is that within 30 seconds of injection, the T wave rises and the ST segment rises (≥0.1mV), which is most obvious in the last 10 seconds. Performance is after 30 seconds: T wave is flat, two-way or reversed, ST segment has no obvious change, P? R and Q? The T period is prolonged, and changes in this stage may last from a few minutes to tens of minutes. This specific ECG change is not only a sign of the success of the model, but also a criterion for evaluating intervention factors (such as drugs). It is believed that the ability to prevent or reduce two-phase changes has a therapeutic effect on model animals. ..: Rats: Choose about 250 g Wistar rats, anaesthetize them by injecting urethane into the abdominal cavity in the supine position, insert electrode needles into the skin of the limbs and chest, and connect to the electrocardiograph for recording. The pituitary injection is injected into the sublingual or tail vein at a dose of 0.5 or 1.0 U/kg. The injection is usually completed within 5 or 10 seconds. Determine the model and intervention effect according to the ECG changes in the rabbit model. [Model Features] This model method has excellent stability and repeatability, is easy to operate, does not need to open the chest cavity, and has low animal mortality. However, coronary artery spasm caused by pituitary injection is non-specific, and there are certain differences in the onset of clinical patients.

　　[Model Evaluation and Application] This model is used in the study of angina pectoris, especially in the study of therapeutic agents for coronary artery spasm and coronary artery dilation. Pituitrin-induced myocardial selectivity is relatively low, the effect of coronary artery contraction is not specific, and the degree and dose of myocardial ischemia vary greatly among animals. When choosing this model, you need to pay attention to these issues.