疾病动物模型,丙型肝炎转基因小鼠模型 z-bio 2021-03-10 473

　　So far, a variety of HCV-related transgenic mouse models have been established, providing a powerful research tool for studying the relationship between HCV constituent genes and liver diseases. However, the phenotypes of transgenic mouse models established in different laboratories are not the same. This section introduces mouse HCV transgenic models.

　　【Modeling method】Express one or more HCV constituent genes in mice and express one or more HCV proteins in mice to study its role in HCV-induced liver disease.

　　[Model Features] The expressed HCV-related gene protein products can be detected in mice, and the liver of some mouse models established in the laboratory also has some pathological changes. In transgenic mice expressing C protein, the researchers found that the liver developed sclerosis, cancerous transformation, apoptosis, and endoplasmic reticulum stress. Co-expression of C and E2 genes, no liver lesions were found. After expressing C-E1-E2, mouse liver also found pathological changes, and even liver cancer occurred. There was no significant difference between C-E1-E2-NS2 expression and non-transgenic control mice. E1-E2-P7 transgenic mice increased the incidence of liver tumors induced by aflatoxin. E1-E2-NS2 transgenic mice can induce cytotoxic T cell response and cause liver damage. NS3/4A transgenic mice can resist liver disease induced by tumor necrosis factor alpha and autophagy induced by interferon. There is no significant difference in liver disease between NS4B transgenic mice and non-transgenic control mice. Different laboratories use NS5A transgenic mice to study liver diseases and find that they are not uniform.

　　[Model Evaluation and Application] Many HCV transgenic mouse models have been developed, and some research results are not consistent. It may be related to different vectors constructed in different laboratories, different promoters used, and different insertion sites and copy numbers. HCV transgenic mice provide a good model for studying the relationship between different HCV constituent genes and liver diseases, and play an important role in studying the relationship between HCV gene-encoded proteins and the host. It should be pointed out that the mice themselves cannot be infected with HCV.

　　gene mice are mostly tolerant to HCV antigens and cannot show specific immune responses, so they are difficult to be used in vaccine research.