疾病动物模型,人源化丙型肝炎小鼠模型 z-bio 2021-03-10 259

　　The researchers transplanted human hepatocytes into immunodeficient mice so that they can be infected with HCV and become a humanized mouse hepatitis C animal model, which is similar to the humanized hepatitis B mouse model. The method used is also similar. Here we also introduce a transgenic mouse expressing human HCV receptor.

　　[Modeling mechanism] The first step for HCV to infect liver cells is to enter liver cells. Because human and mouse liver cells express different receptor molecules, it is difficult for HCV to enter mouse liver cells. In order to break through this obstacle, scientists expressed genes closely related to HCV infection in mice to make them play a biological role and assist the virus to enter mouse liver cells, thus preparing a mouse model of humanized HCV receptors. . Use this humanized mouse for HCV related research.

　　[Modeling method] Construct an adenovirus expression vector co-expressing CD81, SCARB1, CLDN1 and OCLN, inject 100,000,000,000 plaque forming units (PFU) of adenovirus intravenously into mice, and inoculate the mice with 2×10,000,000 TCID50 24 hours later HCV-CRE.

　　[Model Features] This model changes the surface molecules of mouse liver cells so that they can be infected by HCV and enter the cells, which is closer to natural infection.

　　[Model evaluation and application] This model provides a good research tool for studying HCV infection. It proves that people can break the isolation of the host species infected by pathogenic microorganisms through genetic humanization, and expand the scope of use of mice as a research model. But this model also has some problems. The HCV used in this model is artificially modified, and some scholars still question whether the model can be used for antiviral research. Because antiviral therapy against HCV may be specific to humans, it is still debatable whether the response of mouse liver to HCV after only a few genetic modifications is similar to that of human immunity.