疾病动物模型,脂肪肝复合模型 z-bio 2021-03-10 601

　　[Modeling method] By using genetic modification and nutritional factors at the same time, it is possible to create animal models that conform to the laws of human NAFLD, such as ob/ob mice + MCD diet, db/db mice + MCD diet and Abcb11 mice. MCD diet, ApoE mice + HFD, PPARa mice + MCD diet, etc.

　　[Model Features] The compound model can cause insulin resistance, obesity, steatohepatitis and fibrosis. Among them, the db/db mouse + MCD diet model is more severe than the ob/ob mouse + MCD diet model, inflammation and peripheral fibrosis are more serious, and the appearance time of lesions is significantly reduced and related to it. And hyperleptinemia. After induction with MCD diet, the NAFLD performance of PPARa-/- mice was significantly higher than that of the control group.

　　[Model Evaluation and Application] The pathological changes and etiology of existing animal models vary depending on the model method. No model can fully represent the pathological changes and pathophysiological processes of human FLD. Genetic changes in the recombination model can cause damage to fat metabolism and oxidative stress, but human NAFLD is mainly related to genetic susceptibility and insulin resistance. Genetic modification often produces HAC and HCC. Related HCC may be more suitable for studying the mechanism of NAFLD . Since it is not clear how excessive food causes fatty liver, it is not clear which component of food is most likely to cause obesity and fatty liver and regulate energy metabolism. In humans, it is difficult to control all genetic and environmental factors. Therefore, adjusting the diet composition and intake of animal models is a better way to study obesity-related fatty liver diet. However, there are certain differences between obesity and fatty liver caused by the diet of rodents of different species, ages and sexes. For example, the severity of the MCD model and its development speed depend on the sex, strain and type of experimental animals. The composite model is a model that maximizes the complexity of human NAFLD, brings huge pathological changes and has great potential.

　　Researchers need to choose suitable animal models according to their research goals, technical capabilities and laboratory conditions. The selected animal model should be consistent with the research objectives and have a high model replication rate, such as ob /. The ob and db/db mice above are common tools used in the laboratory to study diseases related to metabolism. They are versatile and easy to obtain. In addition, it is necessary to continuously improve modeling technology through pure animal breeding and improved nutritional diet formulas, and it is necessary to use new technologies, new methods and new indicators in animal experiments in a timely manner.