疾病动物模型,免疫性肝硬化动物模型 z-bio 2021-03-10 469

　　[Modeling mechanism] By importing xenogeneic serum, the body's immune regulation is disordered, and immune complex deposits appear in the portal area, causing vasculitis and perivascular inflammation, activating fat storage cells to transform into fibroblasts, and activating immune cells to release cytokines , Further stimulate the proliferation of collagen, resulting in increased fibrous tissue formation and decreased degradation in liver tissue.

　　【Modeling method】After subcutaneous injection of human serum albumin in rats to sensitize, subcutaneous injection of prostaglandin E1 (PGE1) 200μg/time, 2 times/d, followed by tail vein attack injection 2 times, 1st and 3rd times every week 8mg, the rest is 4mg/time, a total of 9 times, 30 days can form liver fibrosis. Porcine serum or bovine serum can also be used.

　　[Model Features] Deformation and necrosis of hepatocytes in the boundary zone of the liver tissue, inflammatory cell infiltration, and hyperplasia of fibrous tissue in the portal area and between the lobules can be seen.

　　[Model Evaluation and Application] This type of model is immune damage, which is close to the formation of human liver fibrosis and cirrhosis. Although the formation rate of fibrosis is not high, the fibrosis is persistent. Repeated intraperitoneal injection of pig serum has been used to study the role of phagocytes, fat storage cells and muscle fibrosis cells in liver fibrosis and the changes of microcirculation during liver fibrosis.