疾病动物模型,胆汁淤积性肝硬化动物模型 z-bio 2021-03-10 1087

　　[Modeling mechanism] By cutting the bile duct or injecting sclerosing agent, it will artificially block the extrahepatic bile duct, cause the bile duct to dilate, cholestasis, and increase the pressure in the bile duct above the blocked site. Intrahepatic blood vessels are compressed by dilated bile ducts and bile overflow, bile pigmentation, degeneration and necrosis in liver cells, fibrous tissue proliferation and spread to the bile ducts, lobules remodeling, leading to liver fibrosis and cirrhosis.

　　[Modeling method] The common bile duct was ligated to the abdomen with silk thread, and 6-0 silk thread was circulated in two positions of the common bile duct at an interval of 1 cm. Animals usually get nourishment. 4 days after the operation. The amount of food is 20-21 grams. Feed at a rate of / d for 6 weeks to prepare the model. It can be used to create experimental biliary liver fibrosis and liver cirrhosis models in dogs, rats, monkeys and other animals.

　　[Model Features] The inflammation is mild, and there is no obvious hepatocyte necrosis in the liver tissue. Except for mild monocyte infiltration into the portal vein, there are very few inflammatory cells. Liver fibrosis develops rapidly. Liver fibrosis develops about 4 weeks after biliary atresia. The spontaneous reversal rate was low, and obvious fibrosis appeared 16 weeks after modeling. The progression of liver fibrosis is obviously related to the increase of serum acidic procollagen peptides.

　　[Model Evaluation and Application] This model is very suitable for studying the anti-liver fibrosis effects of drugs and screening biochemical indicators.