疾病动物模型,种植性肝癌动物模型 z-bio 2021-03-10 461

　　[Modeling mechanism] A liver cancer model formed by transplanting liver cancer tissues, cell lines or other malignant tumors from animals or humans to animals.

　　[Modeling method] The commonly used transplantation sites in this model are the back subcutaneous or liver, abdominal cavity, etc. The transplantation methods are mainly allogeneic transplantation and xenotransplantation. Rats: The only transplantation method for this model may be BERH-2 transplanted rats with spontaneous hepatic sarcoma and Walker-256 rats induced allogeneic transplantation. The construction method usually adopts the method of large-scale tumor transplantation under the liver capsule or intrahepatic injection of cell homogenate, and the left lobe of the liver is selected as the injection site of 1 to 2 cm, which is slightly close to the edge of the liver. After transplantation, attention should be paid to proper hemostasis. Nude mice: Direct inoculation of human liver cancer cell lines (such as Huh7) or cancer tissues into nude mice is xenotransplantation. The common transplantation sites are intrahepatic, subcutaneous and peritoneal transplants. Transplantation methods include tissue block transplantation, suspension transplantation and tissue homogenization transplantation. Mice: After anesthetizing BALB/c mice with pentobarbital, the mouse H22 hepatocellular carcinoma cell line 1×10,000,000 cells/ml was used for 20μl in situ inoculation. Transplanted rabbit VX2 liver cancer model: The VX2 tumor cell line is derived from a squamous cell carcinoma derived from a rabbit papilloma caused by porcine papilloma and was officially established after 72 transplants. The VX2 tumor tissue block was made into a cell suspension, and about 1ml was injected into the inner thigh muscle of the rabbit. Three weeks later, the rabbit with cancer was subjected to general anesthesia, the tumor tissue was peeled off, and the tumor tissue was peeled off. The diameter is as large as possible and is cut into 1 to 2 mm. After general anesthesia, use ophthalmic forceps to open the abdomen and make a hole in the liver tissue of the thick-walled part of the left middle lobe of the liver to form a small opening (3-5 mm) and a large sinus (3-5 mm). I will. 5 to 8mm) cut into 2 to 3 pieces. Transplant the ruptured tumor strain and fill the sinus opening with a piece of gelatin sponge.

　　[Model Features] The transplanted rat liver cancer model has simple domestic replication, simple transplantation methods, high transplant success rate, fast tumor growth, short survival time of mice, stable and uniform tumor biological characteristics, and blood supply for liver cancer Mainly human. The cancerous tissue transplanted into the abdominal cavity grows in large quantities, mainly between the liver and the stomach, and cancerous ascites may appear later. The subcutaneous transplanted tumor is shallow and limited. Tumors inoculated by intrahepatic transplantation are round or oval in the liver, with increased swelling and infiltration, and an increased incidence of ascites. The nude mouse transplantation model retains the characteristic morphology, function and secretion of alpha-fetoprotein (AFP) in human liver cancer. Pathologically, in the rabbit VX2 implantation model, the tumor is a large solid tumor with aggressive growth and abundant blood supply, similar to large liver cancer. [Model evaluation and application] Rat implant models and VX2 implant models are often used in imaging experiments and local intervention therapy. In addition, rat subcutaneous transplantation has a high spontaneous regression rate, and is usually used to study the mechanism of spontaneous tumor regression. Intrahepatic transplantation is an excellent model for studying local treatment of liver cancer. Nude mice are usually used to study the biological characteristics and etiology of liver cancer.