疾病动物模型,非细菌性前列腺炎动物模型 z-bio 2021-12-04 255

　　[Modeling mechanism] Chronic non-bacterial prostatitis may be caused by mycoplasma, chlamydia and autoimmune diseases. The clinical symptoms of chronic bacterial prostatitis are very similar and non-specific. The main symptoms are urgency, dysuria, and urine. Endlessness, pain and swelling in the perineum, etc. Local injection of chemical agents through the prostate causes chemical inflammation. Or use biological agents to stimulate model animals to produce an immune response and produce immune inflammation in the prostate tissue.

　　【Method of Modeling】

　　1. Rats, C57BL/6 mice, a set of conventional surgical instruments, sterile micropipettes, anesthesia supplies, and corresponding chemicals such as carrageenan, Xiaozhiling, formaldehyde, croton oil, glycerin and 2% agar, etc. Or Freund's complete adjuvant (complete Freund's adjuvant, CFA), DTP vaccine.

　　2. Under anesthesia and aseptic conditions, male rats make a midline incision in the lower abdomen to expose the prostate. According to the needs of the experiment, an appropriate amount of inflammatory agent is injected into the prostate with a micropipette. Or mice were injected intraperitoneally with 3mg/ml CFA 0.5ml, the next day after subcutaneous injection of 1mg purified prostate antigen protein, and intraperitoneal injection of DTP vaccine 0.1ml, prostatitis can be formed in 8 weeks.

　　3. Sample collection and observation indicators visually observe the status of lateral lobe prostate disease; weigh the wet weight of the lateral lobe prostate and calculate the relative weight; count the total number of white blood cells and the density of lecithin bodies in the prostatic fluid; the pathological examination of the prostate is based on the infiltration of inflammatory cells in the interstitium of the prostate. The degree of fibroblast proliferation, the size of the glandular cavity of the prostate, and the amount of secretion in the glandular cavity were scored. The degree of inflammatory cell infiltration and fibroblast proliferation in the interstitium of the prostate was scored in 4 grades, and the size of the glandular cavity of the prostate and the amount of secretions in the glandular cavity were scored in 3 grades. The differences in histopathological scores between different treatment groups were compared and statistically analyzed. Determine the drug effect. 【Features of the model】The prostate gland has gained significant weight. Microscopic examination shows that the tissue structure of the prostate has been damaged to varying degrees. Uneven tissue hyperplasia or atrophy, duct dilation or damage, partial basement membrane destruction, increased or decreased secretions, rat prostate interstitial edema and a large number of inflammatory cells such as lymphocytes, monocytes and neutrophil leukocytes Infiltration, the secretion in the gland cavity decreases or disappears, and there are a large number of inflammatory cells and shed glandular epithelial cells.

　　[Model Evaluation and Application] The pathogenesis of this type of animal model is an acute chemical inflammation, with rapid and severe inflammation and pathological response, and even large-scale necrosis of prostate tissue. These manifestations are not consistent with clinical manifestations, and pathological manifestations are very different and lack pathological specificity. Prostate specimens of experimental animals were confirmed to have chronic inflammation pathological changes by pathological section and electron microscope observation, which are similar to the pathological changes of human chronic nonbacterial prostatitis. At the same time, some glands of experimental mice such as thymus, submandibular gland and spleen were observed. , No pathological changes similar to prostate were found, which shows that this experimental method has good specificity. This model can be used to study the pathogenesis, drug screening and drug efficacy evaluation of chronic non-bacterial prostatitis.

　　In addition to the non-bacterial prostatitis model caused by injection of chemical or biological agents, other methods include: ① castration plus estrogen to induce rat prostatitis; ② mouse neonatal dethymic autoimmune prostatitis model; ③ hereditary Spontaneous prostatitis model in aged rats; ④Pelvic floor injection of lactic acid, etc.