疾病动物模型,急性髓性白血病 z-bio 2022-01-10 384

　　[Modeling mechanism] The human acute myeloid leukemia cell line HL-60 is allotransplanted into SCID mice with T and B lymphocyte function combined with immunodeficiency to establish a SCID mouse model with human hematopoietic function. This is the current study of humans The most commonly used animal model of myeloid leukemia.

　　【Modeling method】Prepare HL-60 cell suspension and inject it into the tail vein of SCID mice weighing 15-20g for 4 to 5 weeks. The amount of injected cells is 6×10000000-7×10000000/mouse, and the wound is pressed. Stop bleeding.

　　【Features of the model】①Mice have a rapid onset of disease. Leukemia cells can be seen in the peripheral blood within 3 weeks, and the proportion of peripheral blood leukemia cells can gradually reach 3% to 11%. The diseased mice are randomly selected for bone marrow examination. The leukemia cells have reached the diagnostic ratio of human leukemia (ie >30%). The mice showed symptoms of onset similar to human leukemia, such as poor mental state, decreased activity, unstable gait, slow movement, and decreased appetite. Some mice developed solid tumors in the abdominal cavity. ② SCID mice inoculated intravenously or intraperitoneally with HL-60 cells can quickly spread in the body and develop disease. After intraperitoneal inoculation, tumor cells can directly infiltrate the surrounding tissues and organs; intravenous inoculation is manifested by the infiltration of leukemia cells in various organs of the body, and a large number of leukemia cells appear in the peripheral blood, which is very similar to the clinical manifestations of human leukemia.

　　【Model evaluation and application】This model is currently a widely used animal model for the study of human acute myeloid leukemia. It is mainly used for research on the mechanism and efficacy of disease occurrence and development.