疾病动物模型,光损伤视网膜疾病 z-bio 2021-03-12 283

　　[Modeling mechanism] It is currently believed that retinal damage caused by strong or continuous light is affected by species, region, diet, light harvesting history, and genetic background pretreatment. One of the mechanisms of retinal oxidative light damage. Retinal oxidative damage and sensor cell damage are the result of transcriptional regulation, alteration and activation of enzyme activities, intracellular metal ions, and multiple apoptotic pathways. At the same time, it may depend on the species.

　　[Modeling method] Esfandiari et al. on lighting method and intensity selection. Facts have proved that alternating light and dark reproduction is more in line with the daily life conditions of organisms and can lower the threshold of retinal damage in rats. More serious damage. Choose 8-9 weeks for adult rats, put the rats in a dark environment for 1 week of adaptive feeding, darken them completely for 12 hours before modeling, and then irradiate them with (5000±300) lux intensity light 3 hours. . The rat retina may be severely damaged. According to the activity characteristics of rats, the modeling time is selected from 19:00 pm to 7:00 am the next day.

　　[Model Features] (5000±300) Irradiation with lux light intensity for 1 to 3 hours may reduce the electrophysiological function of the retina. After irradiating light at different times, the change of ERG measurement is related to the length of irradiation time. The longer the irradiation time, the more obvious the change and the greater the damage. The comparison between the experimental groups has obvious statistical significance. The retinal tissue slices of rats in the normal control group have clear structures, the outer disc membranes of the photoreceptors are arranged neatly and regularly, and the inner and outer nuclear layers are tightly arranged and stained evenly. Exposure to strong light can cause obvious damage to the retina. The damage is mainly in the supratemporal quadrant of the rat. Mild damage is a gradual response, and the degree of damage varies from region to region. At different time points after light irradiation, the thickness of each area of retinal ONL is significantly different.

　　[Model Evaluation and Application] Due to different modeling equipment, animal models of light-damaged retinal diseases have different effects. In the experiment, the retina of the experimental animal must be completely exposed to light. When choosing the light intensity, the appropriate light intensity must be selected according to the purpose of the experiment. Joly et al. are in the age of model animals. It is reported that giving juvenile (2 or 4 weeks) and adult (8 weeks) rats the same light intensity can reduce light damage in juvenile rats, but this benefit is gradual. It disappears after 4 weeks, and the modeling environment temperature is also an important factor affecting the experimental results. In our laboratory, we have found that retinal damage is exacerbated at high temperatures.