疾病动物模型,自发性特应性皮炎 z-bio 2021-03-15 291

　　[Modeling mechanism] c/Nga mouse is a kind of famous Japanese dog, which was successfully bred by Koji Kondo of Nagoya University in 1957. Kondo et al. found that these mice develop dermatitis, hair loss, anemia, and glomerulonephritis as they age, and believe that they may be animal models of autoimmune diseases. In 1997, Matsuda et al. re-studied this strain of mice and determined it as an AD animal model. In 1974, DS hairless (DS-NH) mice mutated from inbred DS mice. DS-NH mice can spontaneously develop dermatitis under normal living conditions, which is thought to be related to Staphylococcus aureus infection.

　　[Model Features] Eight weeks later, the ears, cheeks, eyelids, nose and back of Nc/Nga mice showed rough skin, erythema and scratching behavior, and then appeared erosions, exudates, ulcers and abdomen. .. With age, the condition gradually worsened, and reached a peak at 17 weeks. Histological examination showed obvious hyperkeratosis, hyperkeratosis and spinous process hypertrophy, increased number of mast cells in the dermis, and degranulation of eosinophils. Immunohistochemical analysis revealed more CD4 + T cells, macrophages and some CD8 + T cells infiltration. c/Nga mice impair skin barrier function, increase water loss and reduce skin ceramide levels. These characteristics are very similar to human AD. At 9 weeks, DS-NH mice showed obvious flexibility on the curved skin of the face, neck, chest and forelimbs, accompanied by erythema, edema and erosion, and reached a peak at 25 weeks. Mast cells, eosinophils, CD4 + T lymphocytes and CD11b + macrophages infiltrated the skin lesions. Serum IL-4 and IgE levels increased significantly.

　　[Model Evaluation and Application] c/Nga mouse is an ideal AD animal model. However, this model has two disadvantages. One is the low incidence of AD. Usually sensitive hapten stimulation is needed to ensure the appearance of the disease phenotype; secondly, the severity of skin lesions is difficult to quantify. and so. This model is mainly used for research on the etiology of AD, and is more restricted in clinical drug screening. DS-NH mice are not yet recognized as the best mouse model for AD research. The relationship and differences between them and NC mice and AD need to be further studied.