疾病动物模型,鳞状细胞癌模型 z-bio 2021-11-15 622

　　[Modeling mechanism] Inoculation of human SCC tissue or cancer cells in the back, axilla, groin, chest wall or sublingual of immunodeficient mice can locally produce SCC.

　　[Model Features] Currently commonly used animals include nude mice, C3H, ICR, NC, BALB/c, C57BL/6, SCID, NOD/SCID mice. Rats and guinea pigs can also be used for model construction. Someone transplanted tongue flat epithelial cancer tissue into the tongue of nude mice, and found that the lymph node metastasis rate reached 21% at 4 weeks; after 20 weeks, the lymph node metastasis rate increased to 79%. When Tca8ll3 cells were injected into various parts of nude mice (tongue, bottom of mouth, armpit), the cells injected into the bottom of nude mice showed very strong cervical lymph node metastasis. by

　　The heterogeneous tongue SCC model established by New Zealand rabbits has 100% tumorigenesis, 50% and 20% lung metastasis, respectively. Rabbit tongue squamous cell carcinoma in New Zealand is highly malignant, poorly differentiated, and its biological characteristics are very similar to human tongue squamous cell carcinoma. A rabbit tongue SCC model was established by chemical induction, and the primary tumor tissue of the model was transplanted into human nude mice or rabbits to regenerate tumors. It is also possible to construct an overall visualized animal model of squamous cell skin cancer, and the occurrence, development and infiltration of subcutaneous tumors can be clearly observed in the non-invasive state of experimental animals in vivo. [Model Evaluation and Application] The tongue-shaped SCC model is easy to construct, and it is easy to observe the biological characteristics of SCC repeatedly. However, due to the complex anatomy of the rodent neck lymphatic system, the lymphatic drainage area intersects and affects the choice of lymph nodes; the oral injection model affects the animal's diet, therefore, it is suitable for metastasis of metastatic tongue squamous cell carcinoma model. And rodent mechanisms, but it cannot be used as an animal model for quantitative detection of metastatic cancer cells or for drug intervention experiments.

　　The overall visualization of the skin SCC animal model is very simple, easy to implement and has a high success rate. In drug therapy, the dose-effect relationship between the drug and tumor changes can be dynamically observed in real time, and the changes in serology, immunology and other indicators can be immediately obtained. Skin cancer research has important application value.