疾病动物模型,单纯疱疹病毒动物模型 z-bio 2021-10-14 287

　　Herpes simplex virus (herpes simplex virus, HSV) is a DNA virus that can be cultured. It is divided into two types according to the antigenic characteristics, namely HSV1 and HSV2, which can form different animal models under different conditions, such as viruses Type, infected animal, entry route, location of disease, pathological symptoms, observation indicators, primary or recurrent infection.

　　[Modeling mechanism] To create various animal models of HSV infection, the virus solution must first be extracted from cells infected with the virus in vitro, and the same animal and infection route as the experimental model must be selected, and pathogenicity needs to be measured. Inoculation that causes disease in more than 95% of animals is accomplished by inoculating and infecting normal animals following the same route and location. The virus strain can be HSV1 type Sm44 standard strain, HSV2 333 type standard strain, etc. The cultured cells usually use African green monkey kidney Vero cells. Sensitive animals include guinea pigs, mice, rabbits and golden hamsters, depending on the model requirements and the characteristics of the test drug.

　　[Model Features] HSV1 or HSV2 can be used for infection. Remove the skin on both sides of the spine on the back of the guinea pig and hit it with a plum needle. After that, according to the results of the pathogenicity test, the virus solution was added quantitatively, and skin diseases (inflammation, edema, infiltration, papules, chopped up, knots, etc.) were observed every day. The peak period of skin lesions is usually 3 to 5 days, and the whole process is about 10 to 12 days. The model is self-limiting. The different times after infection can be controlled by local or systemic administration. Generally, observation and treatment should not exceed 10 days.

　　[Model Evaluation and Application] Since viruses can only grow and replicate in vivo or in living cells, this model includes cell culture techniques for virus preparation and selection of sensitive animals. Combined with the results of in vitro cell line experiments, the animal model for inducing HSV infection will be used with high reliability and practicability for the study of disease etiology, drug screening and drug efficacy evaluation. The similar pharmacological reactions of HSV and varicella-zoster virus can predict the clinical effect of the drug on herpes simplex, varicella and herpes zoster.