疾病动物模型,寒证热证动物模型 z-bio 2021-03-15 281

　　Cold and heat are two guidelines for distinguishing the nature of diseases. Cold syndrom is mostly caused by exogenous yin and cold, or chronic internal injury, yang depletion, and yin evil. Heat syndrome is mostly caused by exogenous yin and cold. The evil of fiery heat, or the seven emotions that transform the fire, or the improper diet, the accumulation of heat, or yin deficiency and yang hyperactivity. Syndrome types mainly include deficiency-cold syndrome, deficiency-heat syndrome, cold-experimental syndrome, and actual-heat syndrome.

　　[Modeling mechanism] There are many methods for making animal models of cold and heat syndromes, such as cold-heat medicinal method, low-temperature freezing method, qi method, pungent and warm food method, hunger and cold method, etc. The commonly used method is to cause the animals to take cold and heat prescriptions to cause physical changes to appear asthenia-cold syndrome and asthenia-heat syndrome.

　　[Modeling method] Use male rats, weighing 180-220g, and use warming medicine for deficiency-heat syndrome. Aconite: Cinnamon: Dried ginger is made into a water decoction at a ratio of 1:1:1, and the dosage is 20g/( kg·d), the number of days of medication is 14 days; the cold and cold medicine is used for the deficiency-cold syndrome Anemarrhena: gypsum: Phellodendron: Gentiana in a ratio of 1.5:2:1:1.2 to make a water decoction, feed it, and the dosage is 20g/(kg·d) for 21 days.

　　【Features of the model】The heat syndrome rats have been tested and found that their gastric mucosa has obvious damage, atrophy and proliferation changes, and the bleeding rate has increased. The image of the tongue showed that the tongue was reddish, the tongue surface was old, the tongue coating epithelial proliferation accelerated, and the metabolism was vigorous, basically in line with the manifestation of the syndrome of Yin deficiency and heat in TCM. Rats with cold syndrome gradually show the characteristics of dirty hair, hair loss, lethargy, curled up, fatigue, loose stools, diarrhea, and light tongue. Weight gain is slowed, body temperature is lower, food intake is reduced, swimming time is shortened, and plasma cGMP is increased. , CAMP/cGMP has a decreasing trend, ovarian index is significantly reduced, lactate dehydrogenase activity is significantly increased, etc.

　　[Model Evaluation and Application] At present, the models for cold-heat syndrome are mostly limited to the deficiency syndrome, and the indicators often only explain a certain aspect of the problem, lacking integrity. This model is mostly used for pathological research of deficiency-cold and deficiency-heat syndrome.