腭裂模型 z-bio 2021-03-16 243

　　Cleft palate is a common congenital disease. At present, the repair of soft and hard tissue defects and the restoration of the anatomical structure of cleft cleft play an important role in the treatment of left cleft. Due to the special physiological and anatomical characteristics of the cleft palate, the survival and reconstruction effect of bone grafts on the cleft palate is not ideal. Further research on techniques and methods to further improve survival and reconstruction effects through animal research is needed.

　　She Xiaoming et al. established a cleft palate model using Guizhou mini pigs. In the experiment, 3.3-6.6 kg Guizhou mini-pigs that were 3.5 months old were used. After anesthetizing the animal, place the head slightly above the operating table and lie supine. After fixing the limbs with gauze strips, cross-fix the second incisor with gauze strips. After disinfecting the surgical site before using iodine, cover it with a surgical towel. Approximately 7 ml of regular saline containing epinephrine is added to the surgical area (hard pa mucosal bulge is visible after injection); cut with a blade from the midline of the maxillary dog to soft pa, ala bone mucosal periosteum, and peel the skin flaps on both sides by periosteal peeling Facing upwards (be careful not to damage the great nerve and vascular bundle); the distal ala sutures from the canine teeth of the upper jaw to both sides of the horizontal bone horizontal plate have holes in the back intermittently, and the joints are grooved. Using a bone knife, cut the middle pal along a groove about 0.4-0.5 cm wide. Use a small scimitar to cut the nasal mucosa of the front seam from the soft pa, and then cut off the nasal mucosa and the nasal mucosa. During the cutting process, suture the wound on both sides of the defect so that it is wound between the two mucous membranes. After cutting the soft pa, stitch it up. After the operation, the slit became a "V" shape, and the mouth was connected to the nose. Each pig was injected intramuscularly with 1.6 million units of penicillin to prevent infection. Liquid diet and fortification. After waking up, the pig can hear the sound of c-crack.

　　Observation for 9 months after operation. After 15 days of no infection, the wound healed well, the bone surface was wrapped well, and the gaps were intact. Animals have symptoms such as suffocation and left cracking. During the liquid food, you will see food spilling from the nasal cavity.