动物模型,鳞状细胞癌 z-bo 2020-08-04 620

　　[Modeling mechanism] 4-Nitroquinoline-1-oxide (4-nitroquinoline-1-oxide, 4NQO) is the precursor to the action of 4NQO reductase in the body of 4-hydroxyaminoquinoline, a carcinogen body. The H.rasl gene at the GA switch of the mouse chromosome is further metabolized into the final carcinogen 4-acetamidoquinoline-1-oxide through protonation, and finally combined with the nucleophilic structure of the target organ DNA DNA adduct. It produces the 12th codon, destroys the chromosome, and finally leads to squamous cell carcinoma.

　　[Model Features] Low-concentration water-soluble carcinogen 4NQO successfully caused cancer in the mucosa of the upper gastrointestinal tract in mice, from precancerous lesions to slow carcinogenesis, and its histopathological characteristics were similar to humans. Yes. BALB/c mice fed a high concentration of 4NQO drinking water (0.01%) showed rough white plaques, gray ridges, ulcers, erosions, papillary hyperplasia and other mucous membranes on the back of their tongues. I saw the changes and corresponded with simple histology. Epithelial hyperplasia. Mild dysplasia-moderate to severe dysplasia-typical pathological changes of non-invasive cancer, histopathological features are similar to human head and neck squamous cell carcinoma.

　　[Model evaluation and application] This model is a simple method, typical lesions, and has good reproducibility. It is the best animal model for studying the mechanism of head and neck squamous cell carcinoma and screening preventive or therapeutic drugs .