疾病动物模型,酒精性脂肪肝模型 z-bio 2021-11-04 404

　　Animal models of alcoholic fatty liver are divided into two categories: acute fatty liver models and chronic fatty liver models. As you know, alcohol is directly toxic to the liver and is the main cause of liver damage. In order to deeply study the causes of alcoholic fatty liver and to screen effective drugs for the prevention and treatment of alcoholic fatty liver, it is necessary to choose an ideal experimental animal model. At the same time, the model should have similar characteristics to human alcoholic liver disease. Lesions have a specific developmental process, high formation rate, low mortality, simple and easy-to-use modeling methods and lesion reversal. It is very slow after stopping modeling, which is useful for drug intervention research. Some scholars choose miniature pigs to replicate chronic liver injury models to ensure drinking. The experimental method is as follows. Mix 65% corn flour and 35% bile and alcohol into the feed, firstly with carbon tetrachloride [0.2? 0.3 ml/(kg? D)] and phenobarbital sodium [2 mg/(kg? D) )]mixing. Feed a small amount of feed quickly, and then feed the feed without carbon tetrachloride and phenobarbital sodium, diluted to 10% absolute ethanol as the only beverage, and feed once a day to prepare the model. Feed normally for 8 weeks. .. The pathological results at the end of the 4th week showed that steatohepatitis had developed: liver cells were swollen, and lipid droplets of different sizes and irregular distribution appeared in the cytoplasm. The lipid droplets placed the nucleus on the edge of the cell and pushed it in. , Liver cells showed punctate necrosis. Mixed inflammatory cell infiltration appears in the vascular area. After 8 weeks, the pathological results showed liver fibroblasts: the liver cells in the center of the portal vein showed increased hepatocytes, such as large lipid drops, sheet-like hepatocyte necrosis, and lobules, collapsed structure, and reticular fibers gathered in the necrosis. In this area, fibroblasts proliferated in the portal vein and collagen fiber deposits were found in the vein. As a result, the alcoholic fatty liver model was successful in miniature pigs and proved to be replicable.