动物模型,细胞癌 z-bo 2020-08-04 637

　　[Modeling mechanism] Inoculating human SCC tissue or cancer cells on the back, forelimb armpits, inguinal area, lateral chest wall or under the tongue of immunodeficient mice can produce SCC locally.

　　[Model Features] Currently commonly used animals include nude mice, C3H, ICR, NC, BALB/c, C57BL/6, SCID, NOD/SCID mice, etc. In addition, rats, guinea pigs, etc. can also be used for model construction. Someone implanted tongue squamous cell carcinoma tissue into the tongue of nude mice and found that the lymph node metastasis rate was 21% in the fourth week; after 20 weeks, the lymph node metastasis rate rose to 79%. Tca8ll3 cells were injected into different parts of nude mice (tongue, floor of mouth, armpit), and it was found that the cells injected into the floor of mouth of nude mice had strong cervical lymphatic metastasis.

　　Using the non-homogenous tongue SCC model established by New Zealand rabbits, the tumor formation rate was 100%, and the lymph node metastasis rate and lung metastasis rate were 50% and 20%, respectively. The tongue squamous cell carcinoma cells of New Zealand rabbits are highly malignant and poorly differentiated, and their biological characteristics are very similar to those of human tongue squamous cells.

　　Through chemical induction, a rabbit tongue SCC model was established, and the primary tumor tissue of the model was transplanted subcutaneously in nude mice or rabbits to generate tumors again. It is also possible to construct an overall visualized animal model of skin squamous cell carcinoma, and the occurrence, development and infiltration of subcutaneous tumors can be clearly observed in the non-invasive state of experimental animals in vivo.

　　[Model Evaluation and Application] The tongue SCC model is simple to construct, and it is easy to repeatedly observe the biological characteristics of SCC, which has advantages. However, due to the complex anatomical relationship of the lymphatic system in the neck of rodents, the lymphatic drainage area is crisscrossed, which affects the collection of lymph nodes; and the oral injection modeling affects the animal's eating, so the rodent tongue squamous cell carcinoma model is suitable for metastasis and mechanism However, it is not suitable as an animal model for quantitative detection of metastatic cancer cells and drug intervention experiments.

　　The overall visualization of the skin SCC animal model is simple and easy to implement, with a high success rate. In drug therapy, the dose-effect relationship between the drug and tumor changes can be dynamically observed in real time, and the changes of various indicators such as serology and immunology can be obtained in real time, which has important application value for the study of skin cancer treatment.