疾病动物模型,肾外包扎性高血压模型 z-bio 2021-03-22 291

　　A model of extrarenal compressive hypertension with two kidneys and a tie (one kidney is tied and both kidneys are kept), one kidney and one tie (one kidney is tied) (contralateral nephrectomy), and one The tie types that can be divided are divided into two kidneys (both kidneys are bandaged, and both kidneys are retained).

　　(1) The replication method is commonly used in dogs and rats. After conventional anesthesia, fix it, open the abdominal cavity, separate and expose one side of the kidney, wrap it in cellophane and return to the abdominal cavity. The bandage must be tight enough, but not squeeze the tissue. The contralateral kidney can be retained, removed or bandaged, depending on the test requirements. Generally, animals with a systolic blood pressure higher than 160 mmHg (21.3 kPa) after 6 weeks are considered hypertension. Animal surgery usually uses a paravertebral incision on the back, but there are reports advocating the use of a midline abdominal incision. The specific method is as follows.

　　After anesthesia, use a rat weighing 120-150 g, fix it in the prone position, place a punching bag under the abdomen, cut hair in the surgical area, disinfect the skin and trim it along it. Cut the midline skin of the spine from the 10th thoracic vertebra to the 3rd lumbar vertebra. Use small vascular forceps to separate the muscles 1.5-2 cm below the left quarter rib and 1 cm from the spine, and squeeze the kidneys with two fingers. At the bottom of the abdomen, carefully remove the kidney from the surrounding tissue and use sterile cellophane. Bandage the kidney (cellophane is sterilized with ethanol for 2-3 days every night). Pre-cut a small hole in the center of the cellophane to allow the renal artery, vein and ureter to pass through. Cut one side of the cellophane from the center to the edge, wrap the cellophane around the kidney through the edges, and sew the edges of the paper with a mat. You can also cut your own double-layer latex film into an "X" shape, wrap the kidney around the kidney hilum, then cut the right kidney on the opposite side, take out the right kidney, separate and cut..., and finally layer the surgical incision Stitched. After surgery, 1% sodium chloride solution can be added as an accelerator. Twenty days after the operation, more than 70% of the rats suffered from persistent hypertension. Systolic blood pressure can usually rise by 50% or more. The procedure for the dog is basically the same.

　　(2) Model features In addition to cellophane or latex film, the material of the kidney bandage can also be silk, latex, sponge and other materials. Foreign body bandages on the outside of the kidney can cause perinephritis, forming a fibrous sheath outside the kidney, compressing the kidney parenchyma, causing kidney tissue ischemia, increasing the formation of renin, and containing blood angiotensin, which may increase blood volume and blood pressure. (3) Comparative medicine This model is a commonly used hypertension model, and its pathophysiology is similar to human hypertension. Surgery is easier, high blood pressure is stable, and the response to hypertensive drugs is more consistent with the response of hypertensive patients.