动物模型 z-bo 2020-08-04 621

　　Simple herpes simplex virus (herpes simplex virus, HSV) is a cultivable DNA virus, which is classified into HSV1 and HSV2 according to its different antigenic characteristics. Different animal models can be formed under different conditions, such as virus type, infected animal, entry route, place of origin, pathological signs, observation indicators, primary or recurrent infection.

　　[Modeling mechanism] To prepare various animal models of HSV infection, first extract the virus solution from the cells infected with the virus in vitro, select the same animal and infection route as the experimental model, and then select the pathogen. Need to make gender decisions. The vaccination that causes disease in more than 95% of animals is accomplished by inoculating and infecting normal animals following the same route and location. Virus strains include HSV1 type Sm44 standard strain and HSV2 333 type standard strain. The cultured cells usually use African green monkey kidney Vero cells. Sensitive animals include guinea pigs, mice, rabbits and golden hamsters, depending on the requirements of the model and the characteristics of the tested drug.

　　[Model Features] HSV1 or HSV2 can be used for infection. The skin on both sides of the spine on the back of the guinea pig is depilated and tapped with plum needles. Then, according to the results of the pathogenicity test, the virus solution was added quantitatively, and the cause of the skin (inflammation, edema, infiltration, pimples, chopped surface, ab, etc.) was observed every day. The incidence of skin lesions usually peaks in 3-5 days, the whole process takes about 10-12 days, and the model is self-limiting. Different times after infection can be controlled by local or systemic administration, but usually observation and treatment should not exceed 10 days.

　　[Evaluation and application of the model] Since viruses can only grow and replicate in vivo or in living cells, this model includes cell culture techniques for virus preparation and selection of sensitive animals. The HSV-induced animal model is combined with the experimental results of in vitro cell lines, with high reliability and practicality for the study of disease etiology, drug screening and drug efficacy evaluation. The similar pharmacodynamic response of HSV and varicella-zoster virus can predict the clinical efficacy of drugs against herpes simplex, varicella and herpes zoster.