应激性高血压模型,动物造模 z-bio 2021-12-21 402

　　"Unconditioned stimuli (epinephrine, angiotensin, electrical stimulation, etc.) are repeatedly combined with conditioned stimuli to induce hypertension. This can be done by dogs, rats, rabbits and other animals.

　　(1) Breeding methods Before the experiment, the 12-week-old male Wistar rats were subjected to stress stimulation, escape, shouting, tailing and pumping as model animals, and combined with the noise generated by random stimulation pulses, choosing the right foot The electric shock is combined to stimulate the foot to increase the intensity of the electric shock. The stimulation pulse power supply will not cause damage, but it will cause a strong reaction. The current is 0.1-0.8mA, the pulse interval is 20-150ms, the wavelength is 2, 20ms, and the stimulation voltage output is 75-150V (adjustable). The frame and wall of the experimental mouse box are 22cm x 22cm x 16cm plexiglass panels, and the bottom of the box is made of 3mm diameter grid-like copper pipes, the distance between the copper pipes is 1cm, and the pressure source is connected. The noise source was placed in front of the pressure box, not in contact with the mouse box. The rats in the experimental group were once a day. I was stressed for a total of 15 days from 8 am to 10 am and 2 pm until 4 pm. One day before stimulation and 3, 6, 9, 12, and 15 days after the stress stimulation, blood pressure and heartbeat were measured by the tail cuff method, numbers were measured and behavior changes were observed, and models of different stress time were established at the 3, 6, 9, and 9 Under the pressure of 12 and 15 days, the blood pressure of rats increased significantly during the stress process, and rose rapidly to (156.4±14.0) mmHg (1 mmHg = 0.133 kPa) within 6 days. The pressure systolic blood pressure was (150.5±12.0) mmHg on the 9th day. It was relatively stable at this high level, and was (152.4±11.0) mmHg on the 15th day. The heart rate was (406.0±18.0) beats/minute and reached (434.6±20.0 beats)/minute on the third day after stress. It increased significantly to (478±21.0 beats)/minute on the 6th day, and reached (463.0). ±17.0 times/minute

　　(2) The incidence of hypertension animal models of this method is higher than that of the same type of experimental stressors, the time required for stress stimulation is shorter, and the effect of using experimental stressors is better

　　(3) In comparative medicine, electric shock and random noise signals are used as conditioned stimuli. This significantly increases the psychological component of stress, promotes the animal's psychological response, and leads to animal hypertension, similar to hypertension caused by man-made stress. , Repeatable and quantifiable procedures.