动物造模,延髓神经压迫致高血压模型 z-bio 2021-11-01 503

　　(1) Copy method After conventional anesthesia, the trachea was intubated into the adult experimental dog and into the right femoral artery, and the blood pressure was monitored with a multi-channel physiological recorder. Lay the experimental dog on its side and rotate it to the non-operative side 30° to 45°. A straight incision with a length of about 5 cm and a straight incision of about 5 cm along the canine’s posterior inferior cerebral artery reaches the center. Descend to the level of the occipital bone and C2 to expose the occipital crown, and use the mastoid and the drilled part of the occipital bone to form a bone window with a diameter of 1 cm. Expose the cranial nerve group under a microscope and carefully identify it. Carefully separate the vagus nerve, common pharyngeal nerve and accessory nerve, and extend the dorsolateral part that connects the adjacent anterior inferior or posterior cerebellar artery from the lateral medulla to the cerebellum, and then loosen the proximal part from 1-1.5 cm. On the left side of the medulla oblongata, the vagus nerve and brain stem are directly compressed, and a silicon or latex balloon is placed on the outside of the artery to support the artery and prevent it from shifting. The systolic blood pressure (SAP), diastolic blood pressure (DAP), mean arterial pressure (MAP) and heart rate (HR) began to rise 1 hour after the operation, and remained high after 6 weeks. After HR, the increase in SAP after surgery was greater than that in DAP, and it was significantly increased compared to before surgery. MRI examination may show signs of vascular compression in the IX and X cranial nerves and ventrolateral medulla of the model dog. Yes. Cats can also perform this operation.

　　(2) Model features This type of model uses balloons to directly compress the nerves, but now under the microscope, it is directly separated from the anterior inferior cerebellar artery or posterior inferior cerebellar artery near the long medulla and uses a balloon. It is supported and fixed and placed in the left ventrolateral medulla, IX and X cranial nerves REZ to form compression. It can maximize the blood vessels of essential hypertension and left medulla oblongata and IX and X cranial nerves. Stability and repeatability are better than balloon compression equipment and bulbar compression methods.

　　(3) Comparative medicine This model more realistically simulates the etiology of clinical neurogenic hypertension. There is no significant difference in blood pressure and heart rate at each observation stage after surgery, so it has good stability and reliable results. However, the surgery is more complicated and the shape of the dog’s skull is quite different. Spiral CT (SCT) and MR examinations should be performed before surgery to understand the anatomical structure and bone landmarks of the dog’s skull. Guide surgical methods. This type of model has fewer applications.