动物造模,脉粥样硬化动物模型 z-bio 2021-03-22 338

　　(1) Feeding method Observe the New Zealand rabbits weighing 2.0 to 2.5 kg and 3 to 4 months old. After 1 week, they are fed a diet containing 1.5% cholesterol 80 g/(kg?D) and freely drink water. Then feed for 12 weeks. After feeding the animals for 12 weeks, the levels of serum TC and LDL-C increased significantly, and a large amount of yellow-white lipid protruded from the aortic wall into the lumen and connected to the sheet from the proximal end. At the distal end of the aorta, the plaque gradually decreased, and the plaque/intima area ratio was (32.48±3.31)%; the intima of the blood vessel became thicker, the plaque was significantly elevated and contained a large number of foam cells and the number of macrophages was obvious Increased and located in the lower central part of the intimal plaque. Another high-fat prescription: 0.5% cholesterol, 5% lard, 94.5% of the basic diet, feeding for 6-8 weeks can reach 80%-100% of the aortic plaque incidence, and significantly increase serum cholesterol. Add 15% egg yolk powder, 0.5% cholesterol and 5% lard to the feed. After three weeks, deduct the cholesterol in the feed and feed for another 3 weeks to achieve 100% aortic plaque incidence.

　　(2) Model features This model is made by feeding rabbits high fat and high cholesterol. After the model was formed, blood lipids changed significantly. It is a model of hyperlipidemia atherosclerosis. Laboratory animals are easily available, cheap, easy to handle, and very sensitive to disease-inducing diets. Add the right amount of cholesterol to the modeling diet so that the modeling can be successful in a short time. This type of model can be avoided. A common disadvantage of long modeling time is that rabbit modeling is better than laboratory animals such as mice, rabbits, pigs, dogs, and monkeys.

　　(3) The contrast medical model animal rabbits fed with high-fat and high-cholesterol diets can have obvious atherosclerosis after 3-4 months, and the pathological changes are human. The ester metabolism of rabbits is similar, but the main difference of ester metabolism comes from the human body; the anatomical distribution of the lesions is mainly in the thoracic artery, and the coronary artery lesions are mainly in the small arteries of the heart, and it mainly occurs in the large branches of the coronary arteries.