动物造模,动脉粥样硬化动物模型 z-bio 2021-11-06 278

　　(1) Breeding method Male quail, weight 90-110g, age 5-6 weeks. The feed is mixed with 1% cholesterol, 1% lard and 1% soybean oil. After 6 weeks, serum cholesterol levels increased significantly, aortic plaque appeared, aortic intima slightly changed, and some foam cells were visible. After 16 weeks, the intima of the aorta was obviously thickened, there were obvious plaques, a large number of foam cells were visible under the microscope, the intima was obviously thickened, the intima was obviously thickened, fibers, sexual hyperplasia, and cells at the age of 32 Necrosis to 36 weeks. And there is a necrotic zone. The increase in fat in the diet will accelerate atherosclerosis. A diet containing 14% lard, 6% peanut oil and 1% cholesterol for 6-8 weeks will accelerate atherosclerosis.

　　(2) Model features This model uses quail as a model, which is easy to use and has a high molding rate. However, after 32 to 36 weeks of a high-fat diet and 4 to 6 weeks of a basic diet, after stopping the high-fat diet, the aortic intima thickness and plaque score were significantly reduced, that is, compared with the high-fat diet, the fat diet. I stopped eating. After As, it can recover spontaneously, and there is a certain correlation between the modeling time and the degree of disease. At the same time, due to the small size of the animal, it consumes less experimental drugs, and is convenient for blood collection, management, blood collection and administration.

　　(3) Comparative medical model animal quail's aortic lesions are similar to early human fatty plaques. The main lesion is severe at the bifurcation of the artery and confined to the intima. In the experiment, 1% cholesterol and 2% oil were used to induce As plaques in quail. The severity of pathological changes in As plaques is closely related to the modeling time. In the 16th week, the intimal plaque rises and the intima rises. Obviously thickened. The number of smooth muscle cells is reduced, and the nucleus is heavily stained and picnic-like, and its pathological changes are similar to humans.