动物造模,动脉粥样硬化动物模型 z-bio 2021-12-22 305

　　(1) Breeding method Inject 50-75 mg/kg of adrenaline intravenously into macaque monkeys aged 10 to 16 once a week. After stopping adrenaline, give a high-fat diet of 1% cholesterol per 150 g for 6 months. With continuous doses of methylthiouracil in mg, the total modeling time is 16 to 17 months. After modeling, the animal is anesthetized and the chest is opened. First, intracardiac injection of 2 ml heparin and 1% sodium nitrite, insert the cannula into the left ventricular aorta, amputation of the left atrium, pressure of 14.7 to 18.7 kPa in normal saline at 37°C, and then perform 2% in 4 Inject a fixed solution of formaldehyde and 2.5% glutaraldehyde (250ml/kg body weight) at ℃. The brain is removed by craniotomy, and the middle cerebral artery and its branches are removed from the head by 0.5 to 1.5 cm from both sides. After modeling, the animal’s large and medium-sized extracranial arteries have dense atherosclerotic plaques, the middle cerebral artery mitochondria and endoplasmic reticulum are dilated, the gap is enlarged, the intima is locally thickened and the smooth muscle cell intima is increased, and endothelium is occasionally visible. Ulcers, monocyte attachment, intact internal elastic membrane, smooth muscle cells protruding increase in the culture medium, abundant organelles and "secretion" changes, and interstitial increase. There is an endothelial ulcer in the inner membrane of the central branch. If you use a big monkey aged 16 to 2 to model, you can model in the same way after 22 to 23 months.

　　(2) Features of the model This model is made by primate macaques, fed a high-fat diet for 16-28 months. Compared with other animal models, its cardiovascular anatomy and physiological functions are similar to those of humans. It takes a long time to create the model.

　　(3) Comparative Medicine This model uses macaques as model animals. After the model was created, the pathological changes of the middle cerebral artery were consistent with the early changes of human atherosclerosis. The changes in the hyperfine structure of the central branch also indicate that atherosclerosis is developing. Morphological signs of sexual changes in the middle cerebral artery, highly abnormally proliferated smooth muscle cells in the central branch, and large amounts of collagen fibers produced by adult monkeys increase arterial stiffness. With clinical pathology of cerebral arteriosclerosis in middle-aged and elderly people. The changes are consistent.