动物模型 z-bo 2020-08-04 616

　　The skin is the main organ for human fungal invasion. When limited to skin infections, it is only located in the superficial area of 1 to 2mm. The main pathogenic bacteria of superficial skin fungal infections include Epidermophyton, Microsporum, Trichophyton and so on. Guinea pigs are susceptible to dermatophytes. They are commonly used experimental animals for fungal infections on the superficial skin. Rabbits can also be used.

　　[Modeling mechanism] The strains used in the experiment should be selected from standard strains preserved and confirmed by medical fungi preservation units or identified clinical isolates. Before the experiment, a qualitative and quantitative in vitro antifungal efficacy test should be done to determine the lowest drug concentration that can inhibit and kill the growth of fungi. Usually the skin on the back of the trunk is depilated with sandpaper and the skin is inoculated with 1×10,000,000 fungal cells or the amount of infection determined in advance. Usually, symptoms appear within 5-6 days of infection. After local or oral administration, the curative effect is judged by skin lesion changes, fungal microscopy and culture results.

　　[Model Features] This model has a high degree of conformity with the clinic.

　　[Model Evaluation and Application] It is used to evaluate the efficacy of antifungal drugs locally or systemically.