动物造模,急性胃溃疡动物模型 z-bio 2021-12-24 356

　　(1) Copy method The adult rats were fasted for 48 hours, anesthetized with water, the abdominal wall was cut open to expose the stomach, the stomach was pulled out of the abdominal cavity with blunt forceps, and the stomach was ligated at the joint with surgical sutures. Put the pylorus, duodenum and stomach back to their original positions and suture the abdominal wall. After the operation, the animal was put into an iron cage and fasted, without water, and after 15-19 hours, the entire stomach was removed at the cost of blood loss, the gastric juice was collected and centrifuged at 3000/min for 10 minutes, and aspirated Supernatant, measure its volume, and measure its acidity and acidity. Pepsin activity flattens the larger arc of the stomach, observes mucosal ulcer damage, calculates the number of ulcers and ulcer area, converts them into ulcer index, and conducts histomorphological examination.

　　(2) Model characteristics: Model animals have gastric juice accumulation, increased total acidity, increased pepsin activity, and hyperemia and edema of the pregastric mucosa. There are obvious bleeding points and petechiae or necrotic lesions. Under an optical microscope, leukocyte infiltration and tissue necrosis can be seen, and ulcers penetrate deep into the muscle layer.

　　(3) Comparative medicine pyloric ligation is Shay's traditional classic rat gastric ulcer model method. After the pylorus is ligated, the gastric juice remains in the stomach, weakening the defense ability of the stomach wall and leading to the formation of ulcers. The model usually uses the total acidity of gastric juice, pepsin activity, ulcer index and other indicators to determine the disease state. The success rate of model replication is 85% to 100%. It is worth noting that the degree of damage of this model to gastric ulcers is largely related to various factors during surgery. When copying, special attention should be paid to observing indicators, as the time elapsed after fasting and ligation of the animal. Maintain good stability. Among animal models of acute gastric ulcer, this model is also one of the most classic and commonly used methods.